Measurement

Part:BBa_I13512:Design

Designed by: jkm   Group: iGEM04_MIT   (2005-05-31)


Screening plasmid 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1792
    Illegal AgeI site found at 1904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2674
    Illegal SapI site found at 961


Design Notes

Note: This is NOT the most current version of the measurement device of the screening plasmid. The updated version (2.6) is available in the Registry
The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement.
More on the Endy Lab's work with screening plasmids [http://openwetware.org/wiki/Endy:Screening_plasmid here]

Source

References

View the Open Wetware project on screening plasmids at [http://openwetware.org/wiki/Endy:Screening_plasmid MIT's Endy Lab]